Hello, My question is, I am using mobile phase (0.02 M KH2PO4 in water and adjust the pH to 6.8 with tri ethylamine) I got the all 8 stages separated of our scheme the peak shape & resolution is also good but is it ok to use combination of KH2PO4 & TEA for setting pH 6.8. Or I should change the combination for getting pH 6.8. Thanks
Hi Sarah Canon2025
KH₂PO₄ and triethylamine together is a common and effective strategy for creating buffers, especially when a specific pH range is required. Ensure to calibrate your pH carefully and consider the stability and compatibility of the buffer with your experimental setup. Note that TEA is a volatile component and you must store your sample carefully as its evaporation may change the pH of your isolated sample at a later stage
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Primary reason why chromatographic buffers include Ethanolamine is because it can stabilize some enzymes and proteins, enhancing their activity or stability.