Struggling to Stabilize Enzyme Activity During Cold Storage

Biochemistry
1

Hi There

I’m storing purified enzyme samples at –20°C, but I’m seeing a steady drop-in activity within a week. I’ve already tried adding glycerol, but no major improvement.

Could anyone suggest simple stabilizers or buffer tweaks that work well for enzyme preservation in current lab setups?

Thanks in Advance.

8 Likes
2

Check your enzyme for cysteine content. Adding a strong reducing agent like TCEP may help. Storing under liquid nitrogen rather than at -20C will also prevent oxidation and is better for long term storage. Add glycerol to 50% to prevent freeze thaw cycles at -20C.

6 Likes