I am trying to make up fresh picrosirius red solution as the solutions currently in the lab are rather old. The protocol I followed says add 500mg sirius red F3B to 500ml saturated aqueous picric acid. I bought in the saturated picric acid solution and added the dye, however it is not going into solution (presumably because it is already a saturated solution). Apparently this hasn’t been a problem before. Does anyone have any suggestions please? Thanks.
1 Like