How to microdialyze your protein?

Antibody Based Technologies
1

Hi I have this antibody that I need to run on gel. But it’s been purified under high salt conditions (0.5M NaCl). I know that the gel will give me problems if I run it under the high salt. However, I have been told I can microdialyze my protein. but how??

2

Have you considered using a quick-spin column to exchange your buffer?

2 Likes
3

Hi Helix Welcome to the community. Quick Spin columns are certainly good options for desalting the samples. There is one caveat though and that is with desalting columns you can lose Low Molecular weight Globular Proteins (below 30KDa).
Have you noticed the same?