Antibody Purification

[sachinsharma95 (Sachin)]

Hi,

I want to purify my IgG antibody and fear that dialysis will give an unreasonably low yield. More specifically, I want to gid rid of the all the ascites. Any suggestions as to how I should proceed?

Thanks.

[GAGAN (Dr. Gaganjot Singh)]

Hi Sachin

You can use Protein A or Protein G Agarose Fast Flow and purify the IgG selectively.

If you are dualysing samples, there are several easy tricks to increase concentrations during the dialysis itself e.g. using sucrose.