Black dots in cell culture

Cell & Tissue Culture/ Cell Based Assays General Tissue Culture
1

hi,

I am working on APC 10.1 cell lines. there are small black dots in the culture. The cells are healthy, the cell morphology is normal. And there is absence of bacterial and fungal contamination.

Please, suggest what these black dots are ???

the other thing is the cell line is very sticky and cell disaggregation is a problem. I am using trypsin for this purpose, but the cells dont detach after normal 2-5 min. There are sheets/ flakes of cells in media after tryp. I am interested in proteomics, so i am worried about prolonged use of trypsin then normal std time.

Any suggestions?

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2

Can you share a picture? These could be anything varying between an unfiltered harmless FBS or FCS molecular aggregates to troubling bacterial or fungal spores.

Trypsinization is common method of choice for detaching the cells and you have to provide enough time for cells to get detached. Usually cells recover with in 24-48 h window. For your proteomics analysis, you can use a Cell Scraper to get the cells off and if these are to be harvested immediately for analysis than cell scrapers work well.

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