Hello everyone
Has anyone optimized a high-throughput single-cell RNA-seq workflow using combinatorial indexing for granulosa cell populations?
Looking for answers. Pls help.
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Sarah
Directly optimized combinatorial‑indexing scRNA‑seq workflows tailored for granulosa cells do not appear to exist in current literature. However, the methodological tools are available—including sci‑RNA‑seq, scifi‑RNA‑seq, FIPRESCI, and hashing‑based multiplexing approaches—that could potentially be adapted for granulosa cell profiling in the future.
If you’re working in this area, it might be a valuable opportunity to develop a customized protocol applying these high‑throughput combinatorial indexing methods to granulosa cells, as there seems to be a clear gap. I have worked on Granulosa Cells from Ovaries. In case you need basic optimization for the Cell Cultures and allied Techniques for Isolation, Culture and more, let me know. I will be happy to Help.
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