Hi all, I am working with BHK cells. For one of my experiments I need to use a DMEM/F12 without serum (usualy I use 5%FBS). Any one have got an expireance with this kind of studies? How long can I grow the cells without serum.
Thanks
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If you provide your cells with L-glutamine and enough Glucose as source of energy, it should be good. There are many serum free cocktails that can be supplemented to different types of cells
You can request a Cell Culture Media Guide from Thermo (Gibco) here
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