Rare codons and gene expression

Recombinant Gene Expression
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Can anyone answer what cell line contain rare codons for gene expression ?

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Dear HS Raina

Your query doesnt specify if you are looking for a prokaryotic cell line or a Eukaryotic cell line. However, If you’re expressing a gene with rare codons, the best approach depends on your host system:

  • Use Rosetta or CodonPlus strains in E. coli.

  • Codon optimize your gene for expression in mammalian or yeast cells.

  • CodonPlus-RIL*: Provides additional tRNAs for Arg (AGG, AGA), Ile (AUA), and Leu (CUA), specifically to boost expression of genes with rare codons.

  • Insect Systems: Sf9 and Sf21 cells, used with the baculovirus expression system, may handle rare codons better than bacterial systems due to their closer resemblance to eukaryotic expression machinery.

  • Alternatively, you can also consider co-expression of rare tRNAs if codon optimization isn’t an option.

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