Hi all,
I was wondering if anyone had some advice for getting primary mouse skin epithelial cells to adhere. I’ve been able to isolate the cells well enough, but when I place them in a 24 well plate with Poly L-lysine treated coverslips (which has worked great for me in the past with 293T cells), they won’t adhere. Interestingly, they are also no adhereing to the bottom of the wells either so it may not just be an issue of the poly lysine.
Thanks