MHC-peptide/TCR complexes

Biophysics
1

Hello

Antigen recognition, internalization and expression as peptide by MHC class I and class II is fascinating.

What are the methods to study mimic recognition processes and membrane interfaces? I mean surface sensitive techniques.

Thanks

2

Hi Navneet

There are multiple methods to study the membrane interfaces. Some of which I know are:

  1. electron Microscopy where you can check MHC complex and Antigen interactions.
  2. Fluorescence or Confocal Microscopy
  3. Single particle tracking (SPT)
  4. FRET & FRAP (through confocal) if you have access to these techs in your department or common facility, you can utilize these to visualize your interactions