Hello, I wanted to do invitrotranscription using SP6 RNA polymerase for producing antisense RNA. So for that i have added the SP6 RNA polymerase promoter sequence in the Reverse primer end. While doing PCR im getting a bright non-specific band along with my pcr product…Using that i did in-vitro transcription i gnetting RNA of my interest but the yield is low…Can anyone help me out in getting a solution? Will the non-specific band interfere in my results? I will do Dnase treatment before proceeding with my experiments…