# "Unusual Melt Curves Linked to RNA Extraction Contaminants"

**URL:** <https://scientistsolutions.discourse.group/t/unusual-melt-curves-linked-to-rna-extraction-contaminants/1104>\
**Category:** Standard PCR\
**Created:** [August 12, 2025, 9:36am UTC](https://scientistsolutions.discourse.group/t/unusual-melt-curves-linked-to-rna-extraction-contaminants/1104 "2025-08-12T09:36:08Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Lauraripley](https://avatars.discourse-cdn.com/v4/letter/l/96bed5/32.png) [@Lauraripley](https://scientistsolutions.discourse.group/u/Lauraripley)\
**Post date:** [August 12, 2025, 9:36am UTC](https://scientistsolutions.discourse.group/t/unusual-melt-curves-linked-to-rna-extraction-contaminants/1104/1 "2025-08-12T09:36:08Z")

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Hello

Need your help!!

Has anyone seen strange melt curves caused by contaminants from RNA extraction? what could be the underlying reason?

Thanks in advance.

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**Author:** ![Biographix](https://avatars.discourse-cdn.com/v4/letter/b/b9bd4f/32.png) [@Biographix](https://scientistsolutions.discourse.group/u/Biographix)\
**Post date:** [January 24, 2026, 8:19pm UTC](https://scientistsolutions.discourse.group/t/unusual-melt-curves-linked-to-rna-extraction-contaminants/1104/2 "2026-01-24T20:19:10Z")

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A number of factors can cause it. But importantly, a purification step can improve the yield and avoid this issue. You can use either spin columns or reprecipitate and solubilize your sample to bypass the issue.