# Regarding RNAse Treatment

**URL:** <https://scientistsolutions.discourse.group/t/regarding-rnase-treatment/697>\
**Category:** RNA\
**Created:** [January 14, 2025, 8:47pm UTC](https://scientistsolutions.discourse.group/t/regarding-rnase-treatment/697 "2025-01-14T20:47:34Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![BarryArmstrong](https://avatars.discourse-cdn.com/v4/letter/b/8e7dd6/32.png) [@BarryArmstrong](https://scientistsolutions.discourse.group/u/BarryArmstrong)\
**Post date:** [January 14, 2025, 8:47pm UTC](https://scientistsolutions.discourse.group/t/regarding-rnase-treatment/697/1 "2025-01-14T20:47:34Z")

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After plant DNA extraction do i need to add RNase after final resuspension of DNA pellete in TE buffer or autoclaved distilled water? How do I inactivate RNase after this step?

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**Author:** ![ARGERINE](https://avatars.discourse-cdn.com/v4/letter/a/8491ac/32.png) [@ARGERINE](https://scientistsolutions.discourse.group/u/ARGERINE)\
**Post date:** [January 14, 2025, 8:49pm UTC](https://scientistsolutions.discourse.group/t/regarding-rnase-treatment/697/2 "2025-01-14T20:49:32Z")

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Barry

I moved your post here at a more precise location. RNase is typically added after the DNA pellet is resuspended in the final solution, whether it is TE buffer (Tris-EDTA) or autoclaved distilled water.

You can inactivate RNase by adding Proteinase K, which will digest the RNase enzyme. This is typically done at 55–60°C for 15–30 minutes