# Procollagen staining

**URL:** <https://scientistsolutions.discourse.group/t/procollagen-staining/770>\
**Category:** Histology\
**Created:** [January 16, 2025, 9:37am UTC](https://scientistsolutions.discourse.group/t/procollagen-staining/770 "2025-01-16T09:37:17Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Navneet](https://avatars.discourse-cdn.com/v4/letter/n/8e8cbc/32.png) [@Navneet](https://scientistsolutions.discourse.group/u/Navneet)\
**Post date:** [January 16, 2025, 9:37am UTC](https://scientistsolutions.discourse.group/t/procollagen-staining/770/1 "2025-01-16T09:37:17Z")

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Hi everyone

Can anyone help me with any ideas about differentially staining procollagen and collagen fibers (without immunohistochemistry).

Thanks

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**Author:** ![GAGAN](https://yyz2.discourse-cdn.com/flex004/user_avatar/scientistsolutions.discourse.group/gagan/32/16_2.png) [@GAGAN](https://scientistsolutions.discourse.group/u/GAGAN)\
**Post date:** [March 9, 2025, 12:19pm UTC](https://scientistsolutions.discourse.group/t/procollagen-staining/770/2 "2025-03-09T12:19:45Z")

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Navneet

You can use Picrosirius Red Staining (PSR) + Polarized Light Microscopy that stains mature, cross-linked collagen (type I, II, III) red, while procollagen and newly synthesized collagen appear green or yellow under polarized light. You can expect Mature, thicker fibers to appear Bright red/orange Immature/thin fibers (including procollagen) to look Green/yellow This method is not dependent on immunohistochemistry distinguishing mature and immature collagen fibers.