# Preparing Frozen Tissue Sections **URL:** \ **Category:** Sample Preparation\ **Created:** [January 16, 2025, 9:22am UTC](https://scientistsolutions.discourse.group/t/preparing-frozen-tissue-sections/763 "2025-01-16T09:22:55Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![Sarahcanon2025](https://avatars.discourse-cdn.com/v4/letter/s/f04885/32.png) [@Sarahcanon2025](https://scientistsolutions.discourse.group/u/Sarahcanon2025)\ **Post date:** [January 16, 2025, 9:22am UTC](https://scientistsolutions.discourse.group/t/preparing-frozen-tissue-sections/763/1 "2025-01-16T09:22:55Z")
Hi, I have some questions about ‘Preparing Frozen Tissue Sections’. First, If I should immerse the sample into the O.C.T. and freeze the sample in to liquid nitrogen ? second, I want to detect a trans-membrane protein , which I can used to fix the sections, 4%PFA or Acetone? Finally, Is there anybody could give me a protocol about the frozen section. Thanks! ---
**Author:** ![Pundir-nbrc](https://yyz2.discourse-cdn.com/flex004/user_avatar/scientistsolutions.discourse.group/pundir-nbrc/32/131_2.png) [@Pundir-nbrc](https://scientistsolutions.discourse.group/u/Pundir-nbrc)\ **Post date:** [August 21, 2025, 5:42am UTC](https://scientistsolutions.discourse.group/t/preparing-frozen-tissue-sections/763/2 "2025-08-21T05:42:35Z")
Hi, What we do in our lab-firstly cryoprotect our tissue in 30% sucrose to avoid ice crystal formation that may bring artifacts in tissue, later snap freeze in dry ice _**(solid form of carbon dioxide)**_ then embed in OCT. If you still need, I can share a detailed protocol. ---
**Author:** ![Sarahcanon2025](https://avatars.discourse-cdn.com/v4/letter/s/f04885/32.png) [@Sarahcanon2025](https://scientistsolutions.discourse.group/u/Sarahcanon2025)\ **Post date:** [August 21, 2025, 1:54pm UTC](https://scientistsolutions.discourse.group/t/preparing-frozen-tissue-sections/763/3 "2025-08-21T13:54:54Z")
Thank you for advice.. pls share the protocol as well.