# Need Help with Real time PCR

**URL:** <https://scientistsolutions.discourse.group/t/need-help-with-real-time-pcr/585>\
**Category:** qPCR from DNA Template\
**Created:** [January 2, 2025, 10:53am UTC](https://scientistsolutions.discourse.group/t/need-help-with-real-time-pcr/585 "2025-01-02T10:53:44Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Lauraripley](https://avatars.discourse-cdn.com/v4/letter/l/96bed5/32.png) [@Lauraripley](https://scientistsolutions.discourse.group/u/Lauraripley)\
**Post date:** [January 2, 2025, 10:53am UTC](https://scientistsolutions.discourse.group/t/need-help-with-real-time-pcr/585/1 "2025-01-02T10:53:44Z")

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hello All,

Need you help!! When I run kit standards on RotorGene 3000, I get amplification with 100 copies std and not with 10 copies std. How can I make my assay more sensitive?