# Info needed regarding Coating Buffer for ELISA plates

**URL:** <https://scientistsolutions.discourse.group/t/info-needed-regarding-coating-buffer-for-elisa-plates/677>\
**Category:** Antibody Based Technologies\
**Created:** [January 14, 2025, 10:26am UTC](https://scientistsolutions.discourse.group/t/info-needed-regarding-coating-buffer-for-elisa-plates/677 "2025-01-14T10:26:52Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![sachinsharma95](https://avatars.discourse-cdn.com/v4/letter/s/71c47a/32.png) [@sachinsharma95](https://scientistsolutions.discourse.group/u/sachinsharma95)\
**Post date:** [January 14, 2025, 10:26am UTC](https://scientistsolutions.discourse.group/t/info-needed-regarding-coating-buffer-for-elisa-plates/677/1 "2025-01-14T10:26:52Z")

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Hello I am currently using protamine sulfate as my coating buffer…I have used carbonate and PBS coating buffers. it seems like the protamine works better. Does anyone know the benefits of Precoating the ELISA plates overnight? Also are there coating buffers that would work well with DNA that contain Thymine Dimers?

Thanks