# Immunofluorescence **URL:** \ **Category:** Protein Labels\ **Created:** [January 4, 2025, 6:43am UTC](https://scientistsolutions.discourse.group/t/immunofluorescence/599 "2025-01-04T06:43:38Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![Nishan1618](https://avatars.discourse-cdn.com/v4/letter/n/f07891/32.png) [@Nishan1618](https://scientistsolutions.discourse.group/u/Nishan1618)\ **Post date:** [January 4, 2025, 6:43am UTC](https://scientistsolutions.discourse.group/t/immunofluorescence/599/1 "2025-01-04T06:43:38Z")
Hi, My Immunofluorescence slides have a cloudy appearance. Can anyone explain me the reason? The slides do fluoresce, but have a cloudy appearance and the cells (Testis section) are not clearly visible. I use the standard immmunofluorescent procedure and mount using glycerol jelly. When I prepare H & E staining of the same set of tissues, the sections look clear and good. Where could I be going wrong while doing Immunofluorescence? Could the problem be with the tissue itself? I had fixed the tissue in buffered neutral formalin. ---
**Author:** ![Pundir-nbrc](https://yyz2.discourse-cdn.com/flex004/user_avatar/scientistsolutions.discourse.group/pundir-nbrc/32/131_2.png) [@Pundir-nbrc](https://scientistsolutions.discourse.group/u/Pundir-nbrc)\ **Post date:** [August 18, 2025, 4:41pm UTC](https://scientistsolutions.discourse.group/t/immunofluorescence/599/2 "2025-08-18T16:41:35Z")
There may be problem with your mounting media i.e, Glycerol