# HPLC - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

**URL:** https://scientistsolutions.discourse.group/t/hplc-high-performance-liquid-chromatography/642
**Category:** Chromatography
**Created:** [January 13, 2025, 10:06am UTC](https://scientistsolutions.discourse.group/t/hplc-high-performance-liquid-chromatography/642 "2025-01-13T10:06:26Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Sarahcanon2025](https://avatars.discourse-cdn.com/v4/letter/s/f04885/32.png) [@Sarahcanon2025](https://scientistsolutions.discourse.group/u/Sarahcanon2025)
#### Post date: [January 13, 2025, 10:06am UTC](https://scientistsolutions.discourse.group/t/hplc-high-performance-liquid-chromatography/642/1 "2025-01-13T10:06:26Z")

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Hello, My question is, I am using mobile phase (0.02 M KH2PO4 in water and adjust the pH to 6.8 with tri ethylamine) I got the all 8 stages separated of our scheme the peak shape & resolution is also good but is it ok to use combination of KH2PO4 & TEA for setting pH 6.8. Or I should change the combination for getting pH 6.8. Thanks

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### Author: ![ARGERINE](https://avatars.discourse-cdn.com/v4/letter/a/8491ac/32.png) [@ARGERINE](https://scientistsolutions.discourse.group/u/ARGERINE)
#### Post date: [January 13, 2025, 10:03pm UTC](https://scientistsolutions.discourse.group/t/hplc-high-performance-liquid-chromatography/642/2 "2025-01-13T22:03:35Z")

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Hi Sarah Canon2025

KH₂PO₄ and triethylamine together is a common and effective strategy for creating buffers, especially when a specific pH range is required. Ensure to calibrate your pH carefully and consider the stability and compatibility of the buffer with your experimental setup. Note that TEA is a volatile component and you must store your sample carefully as its evaporation may change the pH of your isolated sample at a later stage

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### Author: ![Biographix](https://avatars.discourse-cdn.com/v4/letter/b/b9bd4f/32.png) [@Biographix](https://scientistsolutions.discourse.group/u/Biographix)
#### Post date: [January 14, 2025, 9:17pm UTC](https://scientistsolutions.discourse.group/t/hplc-high-performance-liquid-chromatography/642/3 "2025-01-14T21:17:19Z")

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Primary reason why chromatographic buffers include Ethanolamine is because it can stabilize some enzymes and proteins, enhancing their activity or stability.