# Getting rid of RBCs

**URL:** <https://scientistsolutions.discourse.group/t/getting-rid-of-rbcs/552>\
**Category:** Cell & Tissue Culture/ Cell Based Assays\
**Created:** [December 19, 2024, 6:30pm UTC](https://scientistsolutions.discourse.group/t/getting-rid-of-rbcs/552 "2024-12-19T18:30:00Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![hsraina007](https://avatars.discourse-cdn.com/v4/letter/h/5daacb/32.png) [@hsraina007](https://scientistsolutions.discourse.group/u/hsraina007)\
**Post date:** [December 19, 2024, 6:30pm UTC](https://scientistsolutions.discourse.group/t/getting-rid-of-rbcs/552/1 "2024-12-19T18:30:00Z")

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Hello

I’m trying to make primary culture of larynx tumour tissue. But unfortunately I can’t remove erythrocytes on my flasks. Do you have any idea how to get rid of them?

Thanks

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**Author:** ![ARGERINE](https://avatars.discourse-cdn.com/v4/letter/a/8491ac/32.png) [@ARGERINE](https://scientistsolutions.discourse.group/u/ARGERINE)\
**Post date:** [December 19, 2024, 6:43pm UTC](https://scientistsolutions.discourse.group/t/getting-rid-of-rbcs/552/2 "2024-12-19T18:43:29Z")

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Hi

You can get rid of erythrocytes by mutiple ways

1. Give extra washes with PBS prior to extraction of cells
2. Use a RBC Lysis solution - which usually is a mixture of Ammonium Chloride (155mM), EDTA (0.1mM) and Sodium bicarbonate (10mM)
3. Perform a density gradient centrifugation when you are isolating cells by using Ficoll or Picoll. You’ll get two separate layers with RBC and cells from laryngeal tissue.

Hope this will help you! Let us know if you need any further Help!