# Deglycosylation of heavily glycosylated protein

**URL:** <https://scientistsolutions.discourse.group/t/deglycosylation-of-heavily-glycosylated-protein/598>\
**Category:** Post-Translational Modifications\
**Created:** [January 4, 2025, 6:27am UTC](https://scientistsolutions.discourse.group/t/deglycosylation-of-heavily-glycosylated-protein/598 "2025-01-04T06:27:16Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Nishan1618](https://avatars.discourse-cdn.com/v4/letter/n/f07891/32.png) [@Nishan1618](https://scientistsolutions.discourse.group/u/Nishan1618)\
**Post date:** [January 4, 2025, 6:27am UTC](https://scientistsolutions.discourse.group/t/deglycosylation-of-heavily-glycosylated-protein/598/1 "2025-01-04T06:27:16Z")

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Hi,

I have a problem with LC/MS on heavily glycosylated protein as the sequence coverage is relatively low. Therefore, i will be trying to deglycosylate the protein. What is the best method and where can i can the method from? Will the PGnase F affect the LC/MS result as it may be the enzyme that get sequence rather than my protein.