# Chymotrypsin storage

**URL:** https://scientistsolutions.discourse.group/t/chymotrypsin-storage/788
**Category:** Mapping & Analyzing
**Created:** [January 18, 2025, 7:14am UTC](https://scientistsolutions.discourse.group/t/chymotrypsin-storage/788 "2025-01-18T07:14:16Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![Navneet](https://avatars.discourse-cdn.com/v4/letter/n/8e8cbc/32.png) [@Navneet](https://scientistsolutions.discourse.group/u/Navneet)
#### Post date: [January 18, 2025, 7:14am UTC](https://scientistsolutions.discourse.group/t/chymotrypsin-storage/788/1 "2025-01-18T07:14:16Z")

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Hi everyone

I’m starting to work with Chymotrypsin and have no idea how to keep working solution for long time. For example, i keep Trypsin in freezer at -80. But recommendation for Chymotrypsin solution is keeping for up to one week at +4C. But i cann’t spend all solution at the moment. Could you give advice how to keep aliquots for long time?

Thank you!

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### Author: ![ARGERINE](https://avatars.discourse-cdn.com/v4/letter/a/8491ac/32.png) [@ARGERINE](https://scientistsolutions.discourse.group/u/ARGERINE)
#### Post date: [March 2, 2025, 9:34pm UTC](https://scientistsolutions.discourse.group/t/chymotrypsin-storage/788/2 "2025-03-02T21:34:33Z")

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Navneet

Chymotrypsin is more delicate than trypsin and tends to lose activity over time, especially in solution. You can prepare a stock solution and then aliquot it to smaller volumes. Prefer snap freezing in liquid nitrogen followed with -80C storage. Finally, when you are thawing the Chymotrypsin, use it at 4C instead of RT.