# Antibody Purification

**URL:** <https://scientistsolutions.discourse.group/t/antibody-purification/679>\
**Category:** Antibody Fusion Proteins\
**Created:** [January 14, 2025, 10:54am UTC](https://scientistsolutions.discourse.group/t/antibody-purification/679 "2025-01-14T10:54:20Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![sachinsharma95](https://avatars.discourse-cdn.com/v4/letter/s/71c47a/32.png) [@sachinsharma95](https://scientistsolutions.discourse.group/u/sachinsharma95)\
**Post date:** [January 14, 2025, 10:54am UTC](https://scientistsolutions.discourse.group/t/antibody-purification/679/1 "2025-01-14T10:54:20Z")

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Hi,

I want to purify my IgG antibody and fear that dialysis will give an unreasonably low yield. More specifically, I want to gid rid of the all the ascites. Any suggestions as to how I should proceed?

Thanks.

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**Author:** ![GAGAN](https://yyz2.discourse-cdn.com/flex004/user_avatar/scientistsolutions.discourse.group/gagan/32/16_2.png) [@GAGAN](https://scientistsolutions.discourse.group/u/GAGAN)\
**Post date:** [January 14, 2025, 3:03pm UTC](https://scientistsolutions.discourse.group/t/antibody-purification/679/2 "2025-01-14T15:03:36Z")

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Hi Sachin

You can use Protein A or Protein G Agarose Fast Flow and purify the IgG selectively.

If you are dualysing samples, there are several easy tricks to increase concentrations during the dialysis itself e.g. using sucrose.