# Transcription / Synthesis

**URL:** https://scientistsolutions.discourse.group/c/rna/transcription-synthesis/230.md

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## [About the Transcription / Synthesis category](https://scientistsolutions.discourse.group/t/about-the-transcription-synthesis-category/237)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [In-vitro transcription](https://scientistsolutions.discourse.group/t/in-vitro-transcription/787)

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**Author:** [@Sarahcanon2025](https://scientistsolutions.discourse.group/u/Sarahcanon2025)\
**Replies:** 0\
**Last updated:** [January 18, 2025, 7:02am UTC](https://scientistsolutions.discourse.group/t/in-vitro-transcription/787 "2025-01-18T07:02:41Z")

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Hello, I wanted to do invitrotranscription using SP6 RNA polymerase for producing antisense RNA. So for that i have added the SP6 RNA polymerase promoter sequence in the Reverse primer end. While doing PCR im getting a b…