# Recombinant Gene Expression
**URL:** https://scientistsolutions.discourse.group/c/recombinant-gene-expression/42.md
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## [About the Recombinant Gene Expression category](https://scientistsolutions.discourse.group/t/about-the-recombinant-gene-expression-category/49)
**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0\
**Last updated:** [July 21, 2024, 9:14pm UTC](https://scientistsolutions.discourse.group/t/about-the-recombinant-gene-expression-category/49 "2024-07-21T21:14:13Z")
Discuss Cloning & Restriction Enzymes, E. Coli & Yeast Strains, Mutagenesis, Plasmids / Cosmids / BAC / PAC, Promoters, Reporter Assays, Transformation, Jobs and Meeting Announcements here:
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## [Inclusion Bodies — Troubleshooting Strategies](https://scientistsolutions.discourse.group/t/inclusion-bodies-troubleshooting-strategies/1128)
**Author:** [@Nishan1618](https://scientistsolutions.discourse.group/u/Nishan1618)\
**Replies:** 0\
**Last updated:** [August 26, 2025, 1:54pm UTC](https://scientistsolutions.discourse.group/t/inclusion-bodies-troubleshooting-strategies/1128 "2025-08-26T13:54:40Z")
Hi there Can anyone help! Recombinant proteins in E. coli often end up as inclusion bodies. Beyond lowering temperature or adding chaperones, what methods have worked best for you to improve solubility?? Thanks
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## [Rare codons and gene expression](https://scientistsolutions.discourse.group/t/rare-codons-and-gene-expression/803)
**Author:** [@hsraina007](https://scientistsolutions.discourse.group/u/hsraina007)\
**Replies:** 1\
**Last updated:** [January 25, 2025, 8:08pm UTC](https://scientistsolutions.discourse.group/t/rare-codons-and-gene-expression/803 "2025-01-25T20:08:01Z")
Can anyone answer what cell line contain rare codons for gene expression ?