# Protein Labels

**URL:** https://scientistsolutions.discourse.group/c/protein-detection/protein-labels/214.md

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## [About the Protein Labels category](https://scientistsolutions.discourse.group/t/about-the-protein-labels-category/221)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [Epitope Tag Detection problems](https://scientistsolutions.discourse.group/t/epitope-tag-detection-problems/1190)

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**Author:** [@Biographix](https://scientistsolutions.discourse.group/u/Biographix)\
**Replies:** 1\
**Last updated:** [December 15, 2025, 12:24pm UTC](https://scientistsolutions.discourse.group/t/epitope-tag-detection-problems/1190 "2025-12-15T12:24:15Z")

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Hi everyone I’m encountering a problem detecting an HA epitope tag that I introduced via cloning into my protein of interest. When performing Western blots on lysates from HEK293T cells transfected with my expression co…

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## [Immunofluorescence](https://scientistsolutions.discourse.group/t/immunofluorescence/599)

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**Author:** [@Nishan1618](https://scientistsolutions.discourse.group/u/Nishan1618)\
**Replies:** 1\
**Last updated:** [August 18, 2025, 4:41pm UTC](https://scientistsolutions.discourse.group/t/immunofluorescence/599 "2025-08-18T16:41:35Z")

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Hi, My Immunofluorescence slides have a cloudy appearance. Can anyone explain me the reason? The slides do fluoresce, but have a cloudy appearance and the cells (Testis section) are not clearly visible. I use the standar…

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## [Cd44 high background](https://scientistsolutions.discourse.group/t/cd44-high-background/644)

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**Author:** [@sachinsharma95](https://scientistsolutions.discourse.group/u/sachinsharma95)\
**Replies:** 1\
**Last updated:** [February 26, 2025, 12:12pm UTC](https://scientistsolutions.discourse.group/t/cd44-high-background/644 "2025-02-26T12:12:25Z")

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Hi, I am using BD’s CD44-PE (IM7 clone) at 1:200 (recommended 1:50) with PBS/2% FBS 1mM EDTA and appear to have a high background with MCF7 cells. Ab incubation 15min on ice. Anybody have suggestions? Thanks

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## [Radiolabel protein](https://scientistsolutions.discourse.group/t/radiolabel-protein/602)

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**Author:** [@Lauraripley](https://scientistsolutions.discourse.group/u/Lauraripley)\
**Replies:** 0\
**Last updated:** [January 4, 2025, 7:01am UTC](https://scientistsolutions.discourse.group/t/radiolabel-protein/602 "2025-01-04T07:01:19Z")

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Hello, I want to label recombinant protein with 14C radioisotope. I have 14C-acetic anhydride to label lysin residues and N terminus. The labeling yield was too low (about 1%!!). Could you tell me what buffer to use to g…