# Post-Translational Modifications

**URL:** https://scientistsolutions.discourse.group/c/protein-chemistry/post-translational-modifications/208.md

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## [About the Post-Translational Modifications category](https://scientistsolutions.discourse.group/t/about-the-post-translational-modifications-category/215)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [New form of PTM](https://scientistsolutions.discourse.group/t/new-form-of-ptm/622)

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**Author:** [@Sarahcanon2025](https://scientistsolutions.discourse.group/u/Sarahcanon2025)\
**Replies:** 4\
**Last updated:** [June 30, 2025, 9:05pm UTC](https://scientistsolutions.discourse.group/t/new-form-of-ptm/622 "2025-06-30T21:05:14Z")

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I have a HA-tagged protein that when expressed, show up as a 40kD protein… and there is a faint band about ~43-45kD… this faint band becomes a major band with MG132 addition to transfected cells. So, it looks like ubiqu…

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## [Deglycosylation of heavily glycosylated protein](https://scientistsolutions.discourse.group/t/deglycosylation-of-heavily-glycosylated-protein/598)

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**Author:** [@Nishan1618](https://scientistsolutions.discourse.group/u/Nishan1618)\
**Replies:** 0\
**Last updated:** [January 4, 2025, 6:27am UTC](https://scientistsolutions.discourse.group/t/deglycosylation-of-heavily-glycosylated-protein/598 "2025-01-04T06:27:16Z")

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Hi, I have a problem with LC/MS on heavily glycosylated protein as the sequence coverage is relatively low. Therefore, i will be trying to deglycosylate the protein. What is the best method and where can i can the metho…