# qPCR from DNA Template

**URL:** https://scientistsolutions.discourse.group/c/dna/qpcr-from-dna-template/113.md

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## [About the qPCR from DNA Template category](https://scientistsolutions.discourse.group/t/about-the-qpcr-from-dna-template-category/120)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [Low qPCR Efficiency](https://scientistsolutions.discourse.group/t/low-qpcr-efficiency/1189)

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**Author:** [@Navneet](https://scientistsolutions.discourse.group/u/Navneet)\
**Replies:** 1\
**Last updated:** [November 6, 2025, 10:23pm UTC](https://scientistsolutions.discourse.group/t/low-qpcr-efficiency/1189 "2025-11-06T22:23:32Z")

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I’m experiencing issues with low and inconsistent qPCR efficiency while developing a quantification method for the A subunit of the hydrazine synthase gene (hzsA). I’m using primer pairs from a publication: hzsA\_1597F:…

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## [Stability of cDNA](https://scientistsolutions.discourse.group/t/stability-of-cdna/1055)

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**Author:** [@HarmanAulakh](https://scientistsolutions.discourse.group/u/HarmanAulakh)\
**Replies:** 4\
**Last updated:** [June 2, 2025, 5:12pm UTC](https://scientistsolutions.discourse.group/t/stability-of-cdna/1055 "2025-06-02T17:12:04Z")

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Hi Can anyone tell me, for how long can I store the cDNA without any loss in the stability? can it be stored more than a year? Thanks a lot in advance

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## [RNA quantification](https://scientistsolutions.discourse.group/t/rna-quantification/766)

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**Author:** [@sachinsharma95](https://scientistsolutions.discourse.group/u/sachinsharma95)\
**Replies:** 1\
**Last updated:** [January 16, 2025, 11:35pm UTC](https://scientistsolutions.discourse.group/t/rna-quantification/766 "2025-01-16T23:35:45Z")

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Hi, I am currently trying to quantify extracted RNA from geobacter for use in qPCR. However, i’m not really sure what all the numbers mean from our RNA quantifier (MWG lambda scan 200). It gives A260raw, A260 Pathlength…

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## [Need Help with Real time PCR](https://scientistsolutions.discourse.group/t/need-help-with-real-time-pcr/585)

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**Author:** [@Lauraripley](https://scientistsolutions.discourse.group/u/Lauraripley)\
**Replies:** 0\
**Last updated:** [January 2, 2025, 10:53am UTC](https://scientistsolutions.discourse.group/t/need-help-with-real-time-pcr/585 "2025-01-02T10:53:44Z")

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hello All, Need you help!! When I run kit standards on RotorGene 3000, I get amplification with 100 copies std and not with 10 copies std. How can I make my assay more sensitive?