# Sample Preparation

**URL:** https://scientistsolutions.discourse.group/c/cytology-histology/sample-preparation/100.md

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## [About the Sample Preparation category](https://scientistsolutions.discourse.group/t/about-the-sample-preparation-category/107)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [Preparing Frozen Tissue Sections](https://scientistsolutions.discourse.group/t/preparing-frozen-tissue-sections/763)

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**Author:** [@Sarahcanon2025](https://scientistsolutions.discourse.group/u/Sarahcanon2025)\
**Replies:** 2\
**Last updated:** [August 21, 2025, 1:54pm UTC](https://scientistsolutions.discourse.group/t/preparing-frozen-tissue-sections/763 "2025-08-21T13:54:54Z")

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Hi, I have some questions about ‘Preparing Frozen Tissue Sections’. First, If I should immerse the sample into the O.C.T. and freeze the sample in to liquid nitrogen ? second, I want to detect a trans-membrane protein…

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## [Adherence of cryosections on slides](https://scientistsolutions.discourse.group/t/adherence-of-cryosections-on-slides/760)

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**Author:** [@BarryArmstrong](https://scientistsolutions.discourse.group/u/BarryArmstrong)\
**Replies:** 1\
**Last updated:** [August 18, 2025, 4:39pm UTC](https://scientistsolutions.discourse.group/t/adherence-of-cryosections-on-slides/760 "2025-08-18T16:39:59Z")

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Hi, I am working with intestinal tissue from rat. When I try to stain the tissue sections after taking them on to the slides. They come off from the slides. I have used ultrafrost, superfrost and silanated slides. But no…