# Proliferation / Apoptosis

**URL:** https://scientistsolutions.discourse.group/c/cell-based-assays-tissue-culture/proliferation-apoptosis/92.md

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## [About the Proliferation / Apoptosis category](https://scientistsolutions.discourse.group/t/about-the-proliferation-apoptosis-category/99)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [Hoechst stain for detecting Apoptosis](https://scientistsolutions.discourse.group/t/hoechst-stain-for-detecting-apoptosis/909)

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**Author:** [@Leemark](https://scientistsolutions.discourse.group/u/Leemark)\
**Replies:** 1\
**Last updated:** [April 4, 2025, 10:00pm UTC](https://scientistsolutions.discourse.group/t/hoechst-stain-for-detecting-apoptosis/909 "2025-04-04T22:00:05Z")

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Hello I am doing Hoechst stain to detect apoptosis in my cells. Apoptotic cells have condensed chromatin which would be stained brighter than that of normal healthy cells. However, when cells undergo mitosis, their chro…

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## [Plate bound Anti-CD3 Anti-CD28 protocol](https://scientistsolutions.discourse.group/t/plate-bound-anti-cd3-anti-cd28-protocol/880)

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**Author:** [@Leemark](https://scientistsolutions.discourse.group/u/Leemark)\
**Replies:** 1\
**Last updated:** [February 20, 2025, 1:00pm UTC](https://scientistsolutions.discourse.group/t/plate-bound-anti-cd3-anti-cd28-protocol/880 "2025-02-20T13:00:27Z")

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Hi, I am planning on stimulating T cells with plate bound anti-CD3 and anti-CD28 I know I want to use a concentration of 1ug/ml but I’m having issues finding a protocol to do this. What do I use as a buffer for coating?…

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## [Cultivation of CHO cells without serum?](https://scientistsolutions.discourse.group/t/cultivation-of-cho-cells-without-serum/751)

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**Author:** [@Nishan1618](https://scientistsolutions.discourse.group/u/Nishan1618)\
**Replies:** 0\
**Last updated:** [January 16, 2025, 8:08am UTC](https://scientistsolutions.discourse.group/t/cultivation-of-cho-cells-without-serum/751 "2025-01-16T08:08:01Z")

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HI I want to cultivate CHO cells without using serum (to extract recombinant proteins). I know that I can do so with some kits, and by creating plasmids that make the cells generate recombinant proteins needed for cell s…

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## [MTT based assays for cell proliferation](https://scientistsolutions.discourse.group/t/mtt-based-assays-for-cell-proliferation/742)

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**Author:** [@Danielsuarez1609](https://scientistsolutions.discourse.group/u/Danielsuarez1609)\
**Replies:** 0\
**Last updated:** [January 16, 2025, 7:41am UTC](https://scientistsolutions.discourse.group/t/mtt-based-assays-for-cell-proliferation/742 "2025-01-16T07:41:12Z")

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Hi, I am planning on using a MTT-based assay for assessing cell proliferation/viability. Has anyone used the alternatives XTT or WST-1? If so, which of the three dyes is best?