# Cell Lines

**URL:** https://scientistsolutions.discourse.group/c/cell-based-assays-tissue-culture/cell-lines/91.md

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## [About the Cell Lines category](https://scientistsolutions.discourse.group/t/about-the-cell-lines-category/98)

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**Author:** [@harmandeep1618](https://scientistsolutions.discourse.group/u/harmandeep1618)\
**Replies:** 0

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## [Harvesting HEK cells](https://scientistsolutions.discourse.group/t/harvesting-hek-cells/912)

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**Author:** [@Leemark](https://scientistsolutions.discourse.group/u/Leemark)\
**Replies:** 4\
**Last updated:** [May 6, 2025, 7:51pm UTC](https://scientistsolutions.discourse.group/t/harvesting-hek-cells/912 "2025-05-06T19:51:50Z")

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Hello, Currently I am working with HEK cells and since i have to study a cell surface receptor I am not using Trypsin EDTA for harvesting them. Instead i am using PBS-EDTA(0.02 %). The cells do dislodge after keeping th…

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## [OIL RED STAINING PROBLEMS](https://scientistsolutions.discourse.group/t/oil-red-staining-problems/743)

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**Author:** [@Danielsuarez1609](https://scientistsolutions.discourse.group/u/Danielsuarez1609)\
**Replies:** 0\
**Last updated:** [January 16, 2025, 7:42am UTC](https://scientistsolutions.discourse.group/t/oil-red-staining-problems/743 "2025-01-16T07:42:23Z")

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Hi, I’m trying to stain my 3T3-L1 cells with Oil Red O after differentiation. In light microscopy I clearly see lipid droplets, but the staining with Oil Red O appear negative…and BLUE!!! I would like to know if the BLU…